Effect of Cryoprotective Solutions on Metabolic Activity of Chlorococcum dissectum and Dunaliella salina Cell Cultures

Development of protocols for cryopreservation microalgae Chlorococcum dissectum Korshikov and Dunaliella salina Teodoresco requires selection effective cryoprotectants their concentrations. One the stages choosing optimal concentrations is determining degree toxicity cells at equilibration stage. Solutions dimethyl sulfoxide, ethylene glycol, ethanol, glycerol (5–30%) modified PVS1 PVS2 (50 75%) were used in research. The effect cryoprotective compounds was determined by resazurin reduction test. least damaging on Ch. made a 10% solution glycerol. Treatment samples with ethanol sulfoxide solutions reduced metabolic activity 31–33%, glycol ones did 50%. Incubation 75% PVS1, 50 more than half compared to control. Ethanol most toxic cryoprotectant D. cells. Exposure 25%. PVS not affect studied index.